Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science
Object category:
Elektronische Ressource
Person/Institution:
Publisher:
John Wiley & Sons, Incorporated
Ort:
Newark
Date:
2023
Language:
Englisch
Additional information
Abstract:
Cover -- Title Page -- Copyright -- Contents -- Preface -- About the Authors -- Chapter 1 Introduction to Directed Evolution and Rational Design as Protein Engineering Techniques -- 1.1 Methods and Aims of Directed Enzyme Evolution -- 1.2 History of Directed Enzyme Evolution -- 1.3 Methods and Aims of Rational Design of Enzymes -- References -- Chapter 2 Screening and Selection Techniques -- 2.1 Introductory Remarks -- 2.2 Screening Methods -- 2.3 Selection Methods -- 2.4 Conclusions and Perspectives -- References -- Chapter 3 Gene Mutagenesis Methods in Directed Evolution and Rational Enzyme Design -- 3.1 Introductory Remarks -- 3.2 Directed Evolution Approaches -- 3.2.1 Mutator Strains -- 3.2.2 Error‐Prone Polymerase Chain Reaction (epPCR) -- 3.2.3 Whole Gene Insertion/Deletion Mutagenesis -- 3.2.4 Saturation Mutagenesis as a Privileged Method: Away from Blind Directed Evolution -- 3.2.5 DNA Shuffling and Related Recombinant Gene Mutagenesis Methods -- 3.2.6 Circular Mutation and Other Domain Swapping Techniques -- 3.2.7 Solid‐Phase Combinatorial Gene Synthesis as a PCR‐Independent Mutagenesis Method for Mutant Library Creation -- 3.2.7.1 Introductory Remarks -- 3.2.7.2 The Sloning Approach to Solid‐Phase Gene Synthesis of a Mutant Library: Comparison with the Respective Molecular Biological Saturation Mutagenesis Library -- 3.2.7.3 The Twist Approach to Solid‐Phase Gene Synthesis of a Mutant Library: Comparison with Molecular Biological Saturation Mutagenesis Library -- 3.2.8 Computational Tools and the Role of Machine Learning (ML) in Directed Evolution and Rational Enzyme Design -- 3.2.8.1 Introductory Remarks -- 3.2.8.2 Designing Mutant Libraries and Estimating Library Completeness -- 3.3 Diverse Approaches to Rational Enzyme Design -- 3.3.1 Introductory Remarks.
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Created:
2023-04-12
Last changed:
2023-01-13
Added to portal:
2023-04-12
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